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Protein Purification

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Protein Purification

Theory and Techniques

  • By: K. S. Dayananda

₹625.50 ₹695.00 Save: ₹69.50 (10%)

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ISBN: 9789386243980

Bind: Paperback

Year: 2018

Pages: 232

Size: 8.5 x 11 Inch

Publisher: Viva Books Originals

Sales Territory: Worldwide

Description:

Protein Purification - Theory and Techniques provides a good reference for the techniques and strategies involved in the purification of antibodies and recombinant proteins. The detailed practical guidelines given in the book aim particularly at laboratory scale purification. The book is designed to give the laboratory worker the information needed to undertake the challenge of designing a strategy to produce a purified protein from a crude mixture or cell debris.

The book also reflects personal preferences amongst the multitude of methods that may be used for protein fractionation. There is no single correct way to solve the problem, and probably teams could choose several alternative procedures and come up with a satisfactory solution. The approach and the results are discussed and compared in this book.

Target Audience:

Undergraduates and Postgraduates of Biochemistry, Molecular Biology, Immunology, Genetic Engineering, Biotechnology and Microbiology.

Contents:

Preface

PART 1: PROTEIN PURIFICATION

Chapter 1: Protein Purification

Chapter 2: Preparation

Chapter 3: Three Phase Purification Strategy

Chapter 4: Capture

Chapter 5: Intermediate Purification

Chapter 6: Polishing

Chapter 7: Examples of Protein Purification Strategies

Chapter 8: Sample Storage Conditions

Chapter 9: Principles and Standard Conditions for Purification Techniques

PART II: ANTIBODY PURIFICATION

Chapter 10: Antibody Purification

Chapter 11: Sample Preparation

Chapter 12: Simple, Rapid Purification by Affinity Chromatography

Chapter 13: Immunoprecipitation

Chapter 14: Multi-Step Purification Strategies

Chapter 15: Removal of Specific Contaminants After Initial Purification

Chapter 16: Large-Scale Purification

PART III: RECOMBINANT PROTEINS

Chapter 17: Purification of Recombinant Proteins

Chapter 18: GST Fusion Proteins

Chapter 19: (His)6 Fusion Proteins

Chapter 20: Handling Inclusion Bodies

Chapter 21: Harvesting and Extraction of Recombinant Proteins

Chapter 22: Buffer Exchange and Desalting of Recombinant Proteins

Chapter 23: Simple Purification of other Recombinant Proteins

Chapter 24: Multi-Step Purification of Recombinant Proteins

APPENDIX 1

APPENDIX 2

APPENDIX 3

Index

About the Authors:

K. S. Dayananda is the Head of the Department of Post Graduate Studies in Biochemistry at Padmashree Institute of Information Sciences, Bangalore. He has been teaching biochemistry, molecular biology, immunology and genetic engineering to undergraduates and postgraduates for several years.

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